Endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in vascular smooth muscle.

نویسندگان

  • K Goto
  • Y Kasuya
  • N Matsuki
  • Y Takuwa
  • H Kurihara
  • T Ishikawa
  • S Kimura
  • M Yanagisawa
  • T Masaki
چکیده

Endothelin is a potent endothelium-derived vasoconstrictor peptide recently characterized from porcine and human vascular endothelial cells. Here we provide evidence that endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in porcine coronary artery smooth muscle. The vasoconstrictor action of endothelin is efficiently antagonized by low doses of the dihydropyridine Ca2+-channel blocker nicardipine. Endothelin augments the Ca2+-induced contraction in a high-K+ depolarizing solution, markedly enhances high-threshold Ca2+-channel current on the whole-cell patch clamp recording, and causes a sustained increase in the intracellular Ca2+ that is largely dependent on extracellular Ca2+. These findings suggest that endothelin exerts its vasoconstrictor effect by either directly or indirectly activating the voltage-dependent Ca2+ channel.

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عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 86 10  شماره 

صفحات  -

تاریخ انتشار 1989